system 18 Search Results


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A) SIM imaging of SEPT2-Halo, actin (FastAct_X) and <t>mEmerald-LaminA.</t> Whole-cell and nuclear zoom are mean projections of short ventral z-stacks for actin and septin, and a mean projection of the entire z-stack for laminA. Individual inverted grayscale and merged orthogonal images in (ii and iii) are mean projections with nuclear boundary indicated by dashed cyan line. B) Whole-cell and subnuclear regions of rendered iPALM images. C) Rendered individual fiber (top), rendered orthogonal view (middle), and orthogonal plot of iPALM points from the same fiber (bottom). D) The difference (Δ) between the median actin and median septin height was calculated for 599 fibers from 7 cells, and plotted for each fiber (gray dots) and cell means (green dots) with error bars indicating mean ± SD of cell means.
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A) SIM imaging of SEPT2-Halo, actin (FastAct_X) and <t>mEmerald-LaminA.</t> Whole-cell and nuclear zoom are mean projections of short ventral z-stacks for actin and septin, and a mean projection of the entire z-stack for laminA. Individual inverted grayscale and merged orthogonal images in (ii and iii) are mean projections with nuclear boundary indicated by dashed cyan line. B) Whole-cell and subnuclear regions of rendered iPALM images. C) Rendered individual fiber (top), rendered orthogonal view (middle), and orthogonal plot of iPALM points from the same fiber (bottom). D) The difference (Δ) between the median actin and median septin height was calculated for 599 fibers from 7 cells, and plotted for each fiber (gray dots) and cell means (green dots) with error bars indicating mean ± SD of cell means.
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A) SIM imaging of SEPT2-Halo, actin (FastAct_X) and <t>mEmerald-LaminA.</t> Whole-cell and nuclear zoom are mean projections of short ventral z-stacks for actin and septin, and a mean projection of the entire z-stack for laminA. Individual inverted grayscale and merged orthogonal images in (ii and iii) are mean projections with nuclear boundary indicated by dashed cyan line. B) Whole-cell and subnuclear regions of rendered iPALM images. C) Rendered individual fiber (top), rendered orthogonal view (middle), and orthogonal plot of iPALM points from the same fiber (bottom). D) The difference (Δ) between the median actin and median septin height was calculated for 599 fibers from 7 cells, and plotted for each fiber (gray dots) and cell means (green dots) with error bars indicating mean ± SD of cell means.
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A) SIM imaging of SEPT2-Halo, actin (FastAct_X) and <t>mEmerald-LaminA.</t> Whole-cell and nuclear zoom are mean projections of short ventral z-stacks for actin and septin, and a mean projection of the entire z-stack for laminA. Individual inverted grayscale and merged orthogonal images in (ii and iii) are mean projections with nuclear boundary indicated by dashed cyan line. B) Whole-cell and subnuclear regions of rendered iPALM images. C) Rendered individual fiber (top), rendered orthogonal view (middle), and orthogonal plot of iPALM points from the same fiber (bottom). D) The difference (Δ) between the median actin and median septin height was calculated for 599 fibers from 7 cells, and plotted for each fiber (gray dots) and cell means (green dots) with error bars indicating mean ± SD of cell means.
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A) SIM imaging of SEPT2-Halo, actin (FastAct_X) and <t>mEmerald-LaminA.</t> Whole-cell and nuclear zoom are mean projections of short ventral z-stacks for actin and septin, and a mean projection of the entire z-stack for laminA. Individual inverted grayscale and merged orthogonal images in (ii and iii) are mean projections with nuclear boundary indicated by dashed cyan line. B) Whole-cell and subnuclear regions of rendered iPALM images. C) Rendered individual fiber (top), rendered orthogonal view (middle), and orthogonal plot of iPALM points from the same fiber (bottom). D) The difference (Δ) between the median actin and median septin height was calculated for 599 fibers from 7 cells, and plotted for each fiber (gray dots) and cell means (green dots) with error bars indicating mean ± SD of cell means.
Anti Ck 18 Af 488, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Croda International Plc dioleoyl sn glycero 3 phosphatidylethanolamine n
A) SIM imaging of SEPT2-Halo, actin (FastAct_X) and <t>mEmerald-LaminA.</t> Whole-cell and nuclear zoom are mean projections of short ventral z-stacks for actin and septin, and a mean projection of the entire z-stack for laminA. Individual inverted grayscale and merged orthogonal images in (ii and iii) are mean projections with nuclear boundary indicated by dashed cyan line. B) Whole-cell and subnuclear regions of rendered iPALM images. C) Rendered individual fiber (top), rendered orthogonal view (middle), and orthogonal plot of iPALM points from the same fiber (bottom). D) The difference (Δ) between the median actin and median septin height was calculated for 599 fibers from 7 cells, and plotted for each fiber (gray dots) and cell means (green dots) with error bars indicating mean ± SD of cell means.
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Croda International Plc dioleoyl sn glycero 3 phospho
A) SIM imaging of SEPT2-Halo, actin (FastAct_X) and <t>mEmerald-LaminA.</t> Whole-cell and nuclear zoom are mean projections of short ventral z-stacks for actin and septin, and a mean projection of the entire z-stack for laminA. Individual inverted grayscale and merged orthogonal images in (ii and iii) are mean projections with nuclear boundary indicated by dashed cyan line. B) Whole-cell and subnuclear regions of rendered iPALM images. C) Rendered individual fiber (top), rendered orthogonal view (middle), and orthogonal plot of iPALM points from the same fiber (bottom). D) The difference (Δ) between the median actin and median septin height was calculated for 599 fibers from 7 cells, and plotted for each fiber (gray dots) and cell means (green dots) with error bars indicating mean ± SD of cell means.
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AutoMate Scientific Inc glycine
A) SIM imaging of SEPT2-Halo, actin (FastAct_X) and <t>mEmerald-LaminA.</t> Whole-cell and nuclear zoom are mean projections of short ventral z-stacks for actin and septin, and a mean projection of the entire z-stack for laminA. Individual inverted grayscale and merged orthogonal images in (ii and iii) are mean projections with nuclear boundary indicated by dashed cyan line. B) Whole-cell and subnuclear regions of rendered iPALM images. C) Rendered individual fiber (top), rendered orthogonal view (middle), and orthogonal plot of iPALM points from the same fiber (bottom). D) The difference (Δ) between the median actin and median septin height was calculated for 599 fibers from 7 cells, and plotted for each fiber (gray dots) and cell means (green dots) with error bars indicating mean ± SD of cell means.
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A) SIM imaging of SEPT2-Halo, actin (FastAct_X) and <t>mEmerald-LaminA.</t> Whole-cell and nuclear zoom are mean projections of short ventral z-stacks for actin and septin, and a mean projection of the entire z-stack for laminA. Individual inverted grayscale and merged orthogonal images in (ii and iii) are mean projections with nuclear boundary indicated by dashed cyan line. B) Whole-cell and subnuclear regions of rendered iPALM images. C) Rendered individual fiber (top), rendered orthogonal view (middle), and orthogonal plot of iPALM points from the same fiber (bottom). D) The difference (Δ) between the median actin and median septin height was calculated for 599 fibers from 7 cells, and plotted for each fiber (gray dots) and cell means (green dots) with error bars indicating mean ± SD of cell means.
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Image Search Results


A) SIM imaging of SEPT2-Halo, actin (FastAct_X) and mEmerald-LaminA. Whole-cell and nuclear zoom are mean projections of short ventral z-stacks for actin and septin, and a mean projection of the entire z-stack for laminA. Individual inverted grayscale and merged orthogonal images in (ii and iii) are mean projections with nuclear boundary indicated by dashed cyan line. B) Whole-cell and subnuclear regions of rendered iPALM images. C) Rendered individual fiber (top), rendered orthogonal view (middle), and orthogonal plot of iPALM points from the same fiber (bottom). D) The difference (Δ) between the median actin and median septin height was calculated for 599 fibers from 7 cells, and plotted for each fiber (gray dots) and cell means (green dots) with error bars indicating mean ± SD of cell means.

Journal: bioRxiv

Article Title: Mechanically-induced Septin Networks Protect Nuclear Integrity

doi: 10.64898/2026.01.20.700414

Figure Lengend Snippet: A) SIM imaging of SEPT2-Halo, actin (FastAct_X) and mEmerald-LaminA. Whole-cell and nuclear zoom are mean projections of short ventral z-stacks for actin and septin, and a mean projection of the entire z-stack for laminA. Individual inverted grayscale and merged orthogonal images in (ii and iii) are mean projections with nuclear boundary indicated by dashed cyan line. B) Whole-cell and subnuclear regions of rendered iPALM images. C) Rendered individual fiber (top), rendered orthogonal view (middle), and orthogonal plot of iPALM points from the same fiber (bottom). D) The difference (Δ) between the median actin and median septin height was calculated for 599 fibers from 7 cells, and plotted for each fiber (gray dots) and cell means (green dots) with error bars indicating mean ± SD of cell means.

Article Snippet: For EGFP-Ftractin [ ], EGFP-NM2A (Plasmid #11347; Addgene), 3x-NLS-GFP (Plasmid #58468; Addgene) and mEmerald-LaminA (Plasmid #54139; Addgene) exogenous transfections were performed using Neon electroporation (Invitrogen) with 1x 20 ms pulses of 1600V and 3.5 μ g DNA per 200,000 cells in 100 μ L of reaction.

Techniques: Imaging