system 18 Search Results


cas  (Merck & Co)
86
Merck & Co cas
Cas, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/system+18/pm41642708-370-113-116?v=Merck+%26+Co
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cas - by Bioz Stars, 2026-08
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noc 18  (Dojindo Labs)
93
Dojindo Labs noc 18
Noc 18, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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noc 18 - by Bioz Stars, 2026-08
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plvxmcherry  (Addgene inc)
93
Addgene inc plvxmcherry
Plvxmcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
plvxmcherry - by Bioz Stars, 2026-08
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elisa kit  (R&D Systems)
94
R&D Systems elisa kit
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/system+18/pm29285070-59-10-13?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
elisa kit - by Bioz Stars, 2026-08
94/100 stars
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membranes  (Genesee Scientific)
94
Genesee Scientific membranes
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Membranes, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/system+18/bio_rxiv__2025__02__27__640596-163-0-16?v=Genesee+Scientific
Average 94 stars, based on 1 article reviews
membranes - by Bioz Stars, 2026-08
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pbs t washes  (AutoMate Scientific Inc)
93
AutoMate Scientific Inc pbs t washes
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Pbs T Washes, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/system+18/bio_rxiv__519090-235-1-27?v=AutoMate+Scientific+Inc
Average 93 stars, based on 1 article reviews
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goat anti il 18bp  (R&D Systems)
93
R&D Systems goat anti il 18bp
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Goat Anti Il 18bp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/system+18/pmc05877838-67-18-21?v=R%26D+Systems
Average 93 stars, based on 1 article reviews
goat anti il 18bp - by Bioz Stars, 2026-08
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anti mifnar1  (Bio X Cell)
95
Bio X Cell anti mifnar1
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Anti Mifnar1, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/system+18/bio_rxiv__2025__02__07__637027-196-7-16?v=Bio+X+Cell
Average 95 stars, based on 1 article reviews
anti mifnar1 - by Bioz Stars, 2026-08
95/100 stars
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separate elabscience kit  (Elabscience Biotechnology)
94
Elabscience Biotechnology separate elabscience kit
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Separate Elabscience Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/system+18/10__1016_slash_j__ecoenv__2025__119599-114-18-19?v=Elabscience+Biotechnology
Average 94 stars, based on 1 article reviews
separate elabscience kit - by Bioz Stars, 2026-08
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mouse il 18 elisa kits  (R&D Systems)
93
R&D Systems mouse il 18 elisa kits
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Mouse Il 18 Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse il 18 elisa kits - by Bioz Stars, 2026-08
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il 18 mouse il 18 duoset kit  (R&D Systems)
95
R&D Systems il 18 mouse il 18 duoset kit
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Il 18 Mouse Il 18 Duoset Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 18 mouse il 18 duoset kit - by Bioz Stars, 2026-08
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il 18  (R&D Systems)
94
R&D Systems il 18
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Il 18, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/system+18/pmc06925927-78-10-24?v=R%26D+Systems
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Image Search Results


Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) ELISA of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. IL, interleukin; oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.

Journal: Experimental and therapeutic medicine

Article Title: Oxidized low-density lipoprotein-induced p62/SQSTM1 accumulation in THP-1-derived macrophages promotes IL-18 secretion and cell death.

doi: 10.3892/etm.2017.5221

Figure Lengend Snippet: Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) ELISA of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. IL, interleukin; oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.

Article Snippet: Interleukin-18 (IL-18) levels from culture media was measured with an ELISA kit from R&D Systems, Inc., (cat. no. 7620; Minneapolis, MN, USA), according to the manufacturer's instructions.

Techniques: Transfection, Western Blot, Expressing, Control, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, MTT Assay, Flow Cytometry, Staining, Incubation, Small Interfering RNA